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Zebrafish Research Associate (BS/MS)

The Pathways group is utilizing Zebrafish as a genetic model system for the elucidation of disease-associated pathways, and for the identification of therapeutic targets and bioactive compounds. The candidate will participate in the discovery and genetic validation of candidate targets affecting pathways involved in tissue regeneration. The preferred candidate will have strong skills in molecular and developmental biology, including in vivo and genetic manipulations. We seek a scientist with BS/MS training, who will actively engage in experimental design and interpretation, and who works well in a team environment.

The key responsibilities of the position are as follows:

. Molecular cloning to create gene libraries for in vivo screening
. Targeted cloning projects, site-directed mutagenesis, PCR
. Microinjection of DNA or RNA into zebrafish embryos
. Zebrafish genetics, including genotyping of embryos and adults and chemical and/or genetic screening for new mutants and bioactive compounds
. Analysis of mutant phenotypes, including whole mount in situ hybridizations and antibody stainings; imaging microscopy
. Maintain zebrafish stocks, including animal husbandry, egg collection, embryo phenotyping and maintaining clean tanks
. Maintain general lab supplies and reagents Minimum requirements Minimum:
. B.S/B.A or M.S. degree in a biological science with a minimum 5 years laboratory research experience
. Proficiency in the use of current molecular biology techniques and cloning strategies, quantitative PCR, site-directed mutagenesis, construction of reporter systems, assessment of protein levels using antibody reagents, and the design and use of various expression vectors
. Experience using gene knockdown, over-expression or microinjection technologies, including RNA interference, to assay the function of pathway activity in.
. Excellent organizational, communication and computer skills.
Preferred:
. General knowledge of zebrafish husbandry
. 1+ years experience in a zebrafish laboratory
. Experience working with a model organism: embryology, genetics and developmental biology principles, whole mount gene expression analysis, phenotypic analysis.
. Familiar with microscopy and imaging.